NLRP3 Inflammasome Inducer - Adenosine 5'-triphosphate disodium salt

Adenosine triphosphate (ATP), a potassium efflux agent, can trigger the activation of NLRP3 inflammasome in response to PAMPs, such as LPS and peptidoglycan.

It stimulates the caspase-1-dependent cleavage and secretion of IL-1β from LPS-stimulated cells.

Specifications

Working concentration: 5 mM

CAS number: 987-65-5

Molecular Weight: 551.14

Formula: C₁₀H₁₄N₅O₁₃P₃ .2Na

Purity: ≥99.0% (HPLC)

Solubility: 400 mg/ml (725 mM) in water

Contents

• 1g ATP (adenosine 5’-triphosphate disodium salt) provided lyophilized

 ATP is shipped at room temperature.

 Store at -20°C.

Description

Adenosine triphosphate (ATP), a potassium efflux agent, can trigger the activation of NALP3 inflammasome in response to PAMPs, such as LPS and peptidoglycan.

It stimulates the caspase-1-dependent cleavage and secretion of IL-1β from LPS-stimulated cells [1].

ATP triggers the opening of the non- selective cation channel of the purinergic P2X7 receptor, followed by the gradual opening of a larger pore.

The larger pore is attributed to pannexin-1, which is recruited upon P2X7 receptor activation [2].

Activation of the P2X7 receptor results in potassium efflux which is necessary for activation of the post- translational maturation of IL-1β [3].

References:

1. Mariathasan S. et al., 2006. Cryopyrin activates the inflammasome and ATP. Nature 440;228-32.
2. Locovei S. et al., 2007. Pannexin1 is part of the pore forming unit of the P2X(7) receptor death complex. FEBS Lett. 581(3):483-8.
3. Perregaux d. & Gabel ca., 1994. Interleukin-1β maturation and release in response to ATP and nigericin. J Biol. Chem. 269:15195-15203.

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